2RE1
Crystal structure of aspartokinase alpha and beta subunits
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97923 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 61.806, 61.806, 191.901 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.460 - 2.750 |
| R-factor | 0.23568 |
| Rwork | 0.234 |
| R-free | 0.27618 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.399 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.770 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.115 | 0.115 |
| Number of reflections | 10201 | |
| <I/σ(I)> | 31.47 | 1.98 |
| Completeness [%] | 97.9 | 77 |
| Redundancy | 12.5 | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 289 | 20% PEG 1000, Imidazole, Ca(OAc)2, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
