2RDW
Crystal Structure of Human Glycolate Oxidase in Complex with Sulfate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-05-08 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 143.649, 143.649, 110.461 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.770 - 1.950 |
| R-factor | 0.199 |
| Rwork | 0.197 |
| R-free | 0.23500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gox |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.600 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK (9.2L) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 28.170 | 28.170 | 2.020 |
| High resolution limit [Å] | 1.950 | 4.200 | 1.950 |
| Rmerge | 0.050 | 0.028 | 0.225 |
| Total number of observations | 31242 | 9581 | |
| Number of reflections | 38911 | ||
| <I/σ(I)> | 18.9 | 47.7 | 4.2 |
| Completeness [%] | 92.3 | 91.9 | 86.6 |
| Redundancy | 4.83 | 7.62 | 2.62 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | HEPES,PEG 600, Li2SO4, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
