2RDT
Crystal Structure of Human Glycolate Oxidase (GO) in Complex with CDST
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-01-08 |
| Detector | RIGAKU RAXIS IV++ |
| Spacegroup name | I 4 |
| Unit cell lengths | 96.322, 96.322, 79.194 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.160 - 1.950 |
| R-factor | 0.18 |
| Rwork | 0.177 |
| R-free | 0.22300 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.498 |
| Data scaling software | d*TREK (9.2L) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.160 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.380 | |
| Number of reflections | 25585 | |
| <I/σ(I)> | 3.2 | |
| Completeness [%] | 96.8 | 97.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | HEPES,PEG 600, CDST, pH 7.5, temperature 298K, pH 7.50, VAPOR DIFFUSION, SITTING DROP |
