2RDN
Crystal Structure of PtlH with AKG and ent-1PL bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-14 |
| Detector | ADSC |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.499, 70.106, 48.725 |
| Unit cell angles | 90.00, 92.49, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.350 |
| R-factor | 0.173 |
| Rwork | 0.172 |
| R-free | 0.19600 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.173 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE (2.12) |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 1.400 |
| High resolution limit [Å] | 1.350 | 2.910 | 1.350 |
| Rmerge | 0.052 | 0.024 | 0.446 |
| Number of reflections | 63775 | ||
| <I/σ(I)> | 12.2 | ||
| Completeness [%] | 99.5 | 97.1 | 99.5 |
| Redundancy | 3.2 | 3.3 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | sitting drop | 8.5 | 282 | 100mM Tris, 200mM MgCl2, 20% PEG 3350, pH 8.5, sitting drop, temperature 282K |
