2RDM
Crystal structure of response regulator receiver protein from Sinorhizobium medicae WSM419
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-09-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97930 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 122.593, 58.657, 60.301 |
| Unit cell angles | 90.00, 117.96, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.760 |
| R-factor | 0.18872 |
| Rwork | 0.188 |
| R-free | 0.22006 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.269 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.3.0034) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.820 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Rmerge | 0.046 | 0.560 |
| Number of reflections | 37460 | |
| <I/σ(I)> | 9.8 | 1.4 |
| Completeness [%] | 99.4 | 98.4 |
| Redundancy | 3.8 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 10 | 296 | 0.1M CAPS pH 10.0, 200mM Lithium sulfate, 1.2M Sodium dihydrogen phosphate, 800mM Potassium phosphate, 10% Glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 296K |
