2RDK
Five site mutated Cyanovirin-N with Mannose dimer bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-27 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.205, 38.452, 55.953 |
| Unit cell angles | 90.00, 99.94, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.350 |
| R-factor | 0.185 |
| Rwork | 0.184 |
| R-free | 0.19900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2pys |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.202 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER (1.3.1) |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.450 | 1.400 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.092 | 0.511 |
| Number of reflections | 43019 | |
| <I/σ(I)> | 6.4 | 1.97 |
| Completeness [%] | 94.4 | 91.2 |
| Redundancy | 2.5 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 298 | 30% PEG 8000, 100 MM MGSO4, 2 MM MAN2, VAPOR DIFFUSION, HANGING DROP, temperature 298K, pH 6.00 |
