2RDH
Crystal structure of Staphylococcal Superantigen-Like protein 11
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97907 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.391, 98.152, 79.515 |
| Unit cell angles | 90.00, 91.47, 90.00 |
Refinement procedure
| Resolution | 30.890 - 1.700 |
| R-factor | 0.19461 |
| Rwork | 0.192 |
| R-free | 0.23878 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1m4v |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.426 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.890 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.028 | 0.463 |
| Number of reflections | 86026 | |
| <I/σ(I)> | 3 | |
| Completeness [%] | 94.0 | 71.5 |
| Redundancy | 3.8 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2M PEG3350, 0.2M NaH2PO4, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
