2RD3
Crystal structure of TenA homologue (HP1287) from Helicobacter pylori
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 148.420, 148.420, 233.525 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 125.000 - 2.700 |
| R-factor | 0.23638 |
| Rwork | 0.235 |
| R-free | 0.25761 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1to9 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.643 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 125.000 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.081 | 0.520 |
| Number of reflections | 36057 | |
| <I/σ(I)> | 9.4 | 3.8 |
| Completeness [%] | 99.8 | 99 |
| Redundancy | 9.4 | 8.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.1 M Tris, 1.1 M Lithium sulphate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
