2RCV
Crystal structure of the Bacillus subtilis superoxide dismutase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Detector | MARMOSAIC 300 mm CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 68.369, 84.034, 91.951 |
| Unit cell angles | 99.13, 105.98, 105.58 |
Refinement procedure
| Resolution | 45.900 - 1.600 |
| R-factor | 0.211 |
| Rwork | 0.211 |
| R-free | 0.23000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xuq |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.100 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.900 | 1.700 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.133 | 0.305 |
| Number of reflections | 243111 | |
| <I/σ(I)> | 9 | |
| Redundancy | 2.2 | 1.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 30% w/v polyethylene glycol 4000, 0.2M Magnesium chloride hexahydrate, and 0.1 M Tris hydrochloride, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
