2RCT
Crystal structure of human holo cellular retinol-binding protein II (CRBP-II)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-22 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 35.450, 49.966, 74.296 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.200 |
| R-factor | 0.19408 |
| Rwork | 0.193 |
| R-free | 0.21542 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2rcq |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.054 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.900 | 1.230 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.063 | 0.390 |
| Number of reflections | 41159 | |
| <I/σ(I)> | 15.8 | 3.4 |
| Completeness [%] | 97.8 | 86.1 |
| Redundancy | 5.5 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 0.1 M sodium citrate, 0.2 M sodium/potassium tartrate, 1.4 M ammonium sulfate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
