2RCS
IMMUNOGLOBULIN 48G7 GERMLINE FAB-AFFINITY MATURATION OF AN ESTEROLYTIC ANTIBODY
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-07 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.160, 75.550, 86.560 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.100 |
| R-factor | 0.21 |
| Rwork | 0.210 |
| R-free | 0.25900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | GERMLINE FAB W HAPTEN |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.542 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.170 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.080 * | 0.144 * |
| Total number of observations | 94282 * | |
| Number of reflections | 27752 | |
| <I/σ(I)> | 15.7 | 7.2 |
| Completeness [%] | 97.3 * | 91.2 |
| Redundancy | 3.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 * | 10-15 MG/ML FAB IN 0.1M AMMONIUM ACETATE, 0.1M SODIUM CACODYLATE PH 6.0, 18% PEG 4000, 1% DIOXANE |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10-15 (mg/ml) | |
| 2 | 1 | drop | 100 (mM) | ||
| 3 | 1 | drop | Tris | 10 (mM) | |
| 4 | 1 | drop | methionine | 1 (mM) | |
| 5 | 1 | drop | sodium azide | 1 (mM) | |
| 6 | 1 | drop | EDTA | 0.5 (mM) | |
| 7 | 1 | reservoir | ammonium sulfate | 2.0 (M) |
