2RCF
Carboxysome Shell protein, OrfA from H. Neapolitanus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-09-22 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.933, 86.780, 35.214 |
| Unit cell angles | 90.00, 90.22, 90.00 |
Refinement procedure
| Resolution | 43.400 - 2.150 |
| R-factor | 0.187 |
| Rwork | 0.184 |
| R-free | 0.24300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | CRYSTAL STRUCTURE OF CCML PDB ID 2QW7 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.001 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 90.000 | 90.000 | 2.230 |
| High resolution limit [Å] | 2.150 | 4.630 | 2.150 |
| Rmerge | 0.175 | 0.101 | 0.507 |
| Number of reflections | 20056 | ||
| <I/σ(I)> | 6.1 | ||
| Completeness [%] | 99.7 | 100 | 99 |
| Redundancy | 7.2 | 7.1 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | pH 8.0, vapor diffusion, hanging drop, temperature 298K |
