2RCC
Crystal structure of putative class I ribonucleotide reductase (NP_241368.1) from Bacillus halodurans at 1.90 A resolution
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-26 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.91837, 0.97926 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 175.260, 72.780, 111.770 |
| Unit cell angles | 90.00, 124.90, 90.00 |
Refinement procedure
| Resolution | 28.502 - 1.900 |
| R-factor | 0.184 |
| Rwork | 0.183 |
| R-free | 0.21500 |
| Structure solution method | MAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.613 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.3.0040) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 28.502 | 28.502 | 1.970 |
| High resolution limit [Å] | 1.900 | 4.080 | 1.900 |
| Rmerge | 0.055 | 0.032 | 0.389 |
| Number of reflections | 90521 | 17437 | 17841 |
| <I/σ(I)> | 7.13 | 16 | 1.7 |
| Completeness [%] | 98.2 | 96 | 97 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | NANODROP, 10.0% Glycerol, 5.0% PEG 3000, 30.0% PEG 400, 0.1M HEPES pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
