2RCA
Crystal structure of the NR3B ligand binding core complex with glycine at 1.58 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-23 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.915, 83.597, 145.153 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.300 - 1.580 |
| R-factor | 0.19478 |
| Rwork | 0.192 |
| R-free | 0.23133 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2rcb |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.579 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.3.0038) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.640 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Rmerge | 0.048 | 0.326 |
| Number of reflections | 76724 | |
| <I/σ(I)> | 16.6 | 3.25 |
| Completeness [%] | 92.3 | 91.4 |
| Redundancy | 4.5 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 293 | 0.1 M NaCitrate, 17% PEG 4000, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
