2RC9
Crystal structure of the NR3A ligand binding core complex with ACPC at 1.96 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-15 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.675, 103.552, 59.798 |
| Unit cell angles | 90.00, 95.04, 90.00 |
Refinement procedure
| Resolution | 19.910 - 1.960 |
| R-factor | 0.15921 |
| Rwork | 0.156 |
| R-free | 0.21114 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2rc7 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.450 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.3.0038) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.030 |
| High resolution limit [Å] | 1.960 | 1.960 |
| Rmerge | 0.088 | 0.362 |
| Number of reflections | 43239 | |
| <I/σ(I)> | 8.5 | |
| Completeness [%] | 97.9 | 96.4 |
| Redundancy | 2.9 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.7 | 293 | 0.2 M Mg Acetate, 0.1 M NaCitrate, 14% PEG 3350, pH 5.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
