2RBI
STRUCTURE OF BINASE MUTANT HIS 101 ASN
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 291 |
| Detector technology | IMAGE PLATE |
| Collection date | 1993-10-22 |
| Detector | RIGAKU |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 111.260, 69.150, 33.370 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.200 |
| R-factor | 0.178 * |
| Rwork | 0.178 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | WILD-TYPE BINASE |
| RMSD bond length | 0.015 |
| RMSD bond angle | 0.039 |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((AGROVATA) |
| Phasing software | CCP4 |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.260 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.055 | 0.196 |
| Total number of observations | 51822 * | |
| Number of reflections | 13288 | |
| <I/σ(I)> | 7.5 | 3.8 |
| Completeness [%] | 97.2 | 80.7 |
| Redundancy | 3.9 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 | 291 | VAPOR DIFFUSION METHOD, WITH 12MG/ML BINASE HIS101ASN, 40MM GLYCINE PH 7.5, 8.75% POLYETHYL- ENE GLYCOL MR 10,000 AND 2.5% SATURATED SODIUM CITRATE IN THE HANGING DROP, AND 17.5% PEG 10,000, 60MM GLYCINE PH 7.5 AND 5% SODIUM CITRATE IN WELL. LEFT FOR 2 DAYS AT 18 DEGREES CENTIGRADE., vapor diffusion, temperature 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | binase H101N | 12 (mg/ml) | |
| 2 | 1 | drop | glycine | 40 (mM) | |
| 3 | 1 | drop | PEG10000 | 17.5 (%) | |
| 4 | 1 | drop | sodium citrate | 2.5 (%sat) | |
| 5 | 1 | reservoir | PEG10000 | 17.5 (%) | |
| 6 | 1 | reservoir | glycine | 60 (mM) | |
| 7 | 1 | reservoir | sodium citrate | 5 (%) |
