2RBH
Gamma-glutamyl cyclotransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-07-07 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 34.926, 99.406, 99.948 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.970 - 2.100 |
| R-factor | 0.20326 |
| Rwork | 0.201 |
| R-free | 0.25469 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2pn7 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.231 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.170 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.083 | 0.268 |
| Number of reflections | 16603 | |
| <I/σ(I)> | 10.04 | 3.93 |
| Completeness [%] | 78.8 | 78 |
| Redundancy | 2.2 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.25 | 277.15 | 0.1M potassium acetate buffer pH 4.25, 20% PEG 4000, 0.2M ammonium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 277.15K |
