2RBD
CRYSTAL STRUCTURE OF A PUTATIVE SPORE COAT PROTEIN (BH2358) FROM BACILLUS HALODURANS C-125 AT 1.54 A RESOLUTION
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-26 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.91837, 0.97898, 0.97922 |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 43.082, 74.345, 235.812 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.809 - 1.540 |
| R-factor | 0.16 |
| Rwork | 0.159 |
| R-free | 0.18800 |
| Structure solution method | MAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.452 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 39.809 | 39.809 | 1.620 |
| High resolution limit [Å] | 1.540 | 4.870 | 1.540 |
| Rmerge | 0.075 | 0.025 | 0.680 |
| Total number of observations | 10813 | 49670 | |
| Number of reflections | 56674 | ||
| <I/σ(I)> | 9.3 | 23.1 | 1.1 |
| Completeness [%] | 100.0 | 99.7 | 99.9 |
| Redundancy | 6.1 | 5.5 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.1 | 277 | NANODROP, 0.2M Li3 Citrate, 20.0% PEG 3350, No Buffer pH 8.1, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
