2RBC
Crystal structure of a putative ribokinase from Agrobacterium tumefaciens
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-20 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.97873, 0.97886 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 81.024, 81.024, 139.799 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.170 - 1.900 |
| R-factor | 0.19 |
| Rwork | 0.188 |
| R-free | 0.22800 |
| Structure solution method | MAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.490 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.059 | 0.235 |
| Number of reflections | 69844 | |
| <I/σ(I)> | 9.9 | 6.3 |
| Completeness [%] | 99.8 | 98.4 |
| Redundancy | 7 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 292 | 0.1M Bis-Tris pH 6.5, 1.5M Ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
