2RB7

Crystal structure of co-catalytic metallopeptidase (YP_387682.1) from Desulfovibrio desulfuricans G20 at 1.60 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-06-03
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91837, 0.97901, 0.97926
Spacegroup name	P 1 21 1
Unit cell lengths	53.850, 82.190, 89.340
Unit cell angles	90.00, 91.37, 90.00

Refinement procedure

Resolution	27.995 - 1.600
R-factor	0.155
Rwork	0.154
R-free	0.18000
Structure solution method	MAD
RMSD bond length	0.017
RMSD bond angle	1.624
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	27.995	27.995	1.660
High resolution limit [Å]	1.600	3.440	1.600
Rmerge	0.045	0.025	0.314
Number of reflections	101945
<I/σ(I)>	9.92	23.9	2.2
Completeness [%]	98.7	97.8	94.5
Redundancy	3.28

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.1	277	NANODROP, 0.2M Sodium fluoride, 20.0% PEG 3350, No Buffer pH 7.1, VAPOR DIFFUSION, SITTING DROP, temperature 277K