2RB6
X-Ray structure of the protein Q8EI81. Northeast Structural Genomics Consortium target SoR78A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-09-07 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97900 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 49.880, 109.354, 27.070 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.920 - 2.500 |
| R-factor | 0.241 |
| Rwork | 0.241 |
| R-free | 0.28000 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SnB |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.091 | 0.562 |
| Number of reflections | 14617 | |
| <I/σ(I)> | 19.7 | 5.3 |
| Completeness [%] | 98.8 | 99.3 |
| Redundancy | 50.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH UNDER OIL | 6 | 293 | 0.1M NaNO3, 0.1M MES, 20% PEG 4000, pH 6.0, MICROBATCH UNDER OIL, temperature 293K |
