2RAO
X ray crystal structure of rabbit hemoglobin (oxy form) at 2.0 angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-03-03 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 54.608, 54.862, 64.021 |
| Unit cell angles | 67.23, 66.35, 60.68 |
Refinement procedure
| Resolution | 27.300 - 2.000 |
| R-factor | 0.17386 |
| Rwork | 0.171 |
| R-free | 0.21911 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.635 |
| Data reduction software | AUTOMAR |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.500 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 35152 | |
| <I/σ(I)> | 6.9 | 1.4 |
| Completeness [%] | 92.7 | 91.5 |
| Redundancy | 2.47 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | PEG 3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP |
