2RAI

The PX-BAR membrane remodeling unit of Sorting Nexin 9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	CCD
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97952
Spacegroup name	P 21 21 21
Unit cell lengths	65.632, 117.550, 145.814
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.760 - 3.200
R-factor	0.2331
Rwork	0.233
R-free	0.30100
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.200
Data reduction software	XDS
Data scaling software	XDS
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	19.900
High resolution limit [Å]	3.200
Rmerge	0.069
Number of reflections	35702
<I/σ(I)>	21.5
Completeness [%]	99.2
Redundancy	7.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	1.3M Ammonium tartrate dibasic, 0.1M BIS-TRIS propane, pH 7.0 , VAPOR DIFFUSION, HANGING DROP, temperature 293K