2RA9

Crystal structure of a duf1285 family protein (sbal_2486) from shewanella baltica os155 at 1.40 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2007-08-30
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9795, 0.9798, 1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	38.409, 62.286, 73.247
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.854 - 1.400
R-factor	0.164
Rwork	0.162
R-free	0.19600
Structure solution method	MAD
RMSD bond length	0.014
RMSD bond angle	1.519
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHARP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.854	29.854	1.440
High resolution limit [Å]	1.400	6.260	1.400
Rmerge	0.058	0.037	0.613
Total number of observations		1966	11613
Number of reflections	35222
<I/σ(I)>	7.8	17.4	1.3
Completeness [%]	99.6	93.3	98.5
Redundancy	4.9	4.7	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	277	NANODROP, 20.0% Isopropanol, 20.0% PEG 4000, 0.1M Citrate pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 277K