2RA6
Crystal Structure of the Possum Milk Whey Lipocalin Trichosurin at pH 4.6 with Bound 4-ethylphenol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 113 |
| Detector technology | CCD |
| Collection date | 2003-09-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.89 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.318, 100.470, 84.702 |
| Unit cell angles | 90.00, 90.02, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.500 |
| Rwork | 0.226 |
| R-free | 0.24100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2r74 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.179 |
| Data scaling software | XSCALE |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.600 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.072 | 0.289 |
| Number of reflections | 117706 | |
| <I/σ(I)> | 11.6 | 4.3 |
| Completeness [%] | 99.5 | 96 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 291 | 100mM Sodium Acetate, 200mM ZnCl2, 8% 2-propanol, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
