2RA5
Crystal structure of the putative transcriptional regulator from Streptomyces coelicolor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-03-21 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97920 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 43.768, 43.768, 170.735 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.680 - 2.400 |
R-factor | 0.225 |
Rwork | 0.218 |
R-free | 0.29200 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.804 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.384 | |
Number of reflections | 6859 | |
<I/σ(I)> | 8.3 | 2.2 |
Completeness [%] | 96.3 | 75.2 |
Redundancy | 20.8 | 7.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | 0.1M Tris-HCl pH 8.5, 0.2M MgCl2, 20% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 295K |