2R9U
Crystal Structure of Lamprey Variable Lymphocyte Receptor 2913 Ectodomain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-03 |
| Detector | MarUSA MarMosaic -325 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.109, 61.057, 98.081 |
| Unit cell angles | 90.00, 107.35, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.100 |
| R-factor | 0.22 |
| Rwork | 0.217 |
| R-free | 0.26400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2o6r |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.654 |
| Data reduction software | MOSFLM |
| Data scaling software | HKL-2000 |
| Phasing software | CaspR |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 43844 | |
| <I/σ(I)> | 9 | 3 |
| Completeness [%] | 91.3 | 81.3 |
| Redundancy | 3.1 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 295 | 2.0-2.4 M NaH2PO4/K2HPO4, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
