2R9Q
Crystal structure of 2'-deoxycytidine 5'-triphosphate deaminase from Agrobacterium tumefaciens
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-08-22 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97940, 0.97960 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.693, 129.042, 85.559 |
| Unit cell angles | 90.00, 108.75, 90.00 |
Refinement procedure
| Resolution | 46.780 - 2.200 |
| R-factor | 0.1898 |
| Rwork | 0.189 |
| R-free | 0.26949 |
| Structure solution method | MAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.521 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.780 | 2.260 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.099 | 0.647 |
| Number of reflections | 69099 | |
| <I/σ(I)> | 17.1 | 0.8 |
| Completeness [%] | 93.9 | 53.36 |
| Redundancy | 4.1 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 295 | 0.2M CaCl2, 20% PEG 3350, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
