2R98
Crystal Structure of N-acetylglutamate synthase (selenoMet substituted) from Neisseria gonorrhoeae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2007-06-18 |
| Wavelength(s) | 0.97472 |
| Spacegroup name | P 3 1 2 |
| Unit cell lengths | 98.975, 98.975, 89.292 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.400 |
| R-factor | 0.19694 |
| Rwork | 0.194 |
| R-free | 0.24394 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 2.070 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.540 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.092 | 0.684 |
| Number of reflections | 19707 | |
| <I/σ(I)> | 33.8 | 2.2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 9.2 | 8.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 291 | 6% PEG3350, 100mM CsCl, 100 mM sodium citrate, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
