2R90

Crystal structure of cameline peptidoglycan recognition protein at 2.8A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	291
Detector technology	IMAGE PLATE
Collection date	2007-06-15
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.54132
Spacegroup name	I 2 2 2
Unit cell lengths	89.849, 102.370, 164.111
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.730 - 2.800
R-factor	0.205
Rwork	0.205
R-free	0.22000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1yck
RMSD bond length	0.014
RMSD bond angle	2.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.730	2.900
High resolution limit [Å]	2.800	2.800
Number of reflections	18117
Completeness [%]	95.2	92.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8	298	0.2M Sodium Tartarate, 20% PEG3350, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K