2R8Z

Crystal structure of YrbI phosphatase from Escherichia coli in complex with a phosphate and a calcium ion

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Detector technology	CCD
Collection date	2007-02-02
Detector	MARMOSAIC 225 mm CCD
Spacegroup name	P 1 21 1
Unit cell lengths	85.370, 156.908, 114.051
Unit cell angles	90.00, 96.54, 90.00

Refinement procedure

Resolution	30.000 - 2.100
R-factor	0.1863
Rwork	0.184
R-free	0.23075
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.045
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall
Low resolution limit [Å]	38.400
High resolution limit [Å]	2.100
Number of reflections	162029

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP			VAPOR DIFFUSION, HANGING DROP