2R8W
The crystal structure of dihydrodipicolinate synthase (Atu0899) from Agrobacterium tumefaciens str. C58
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-28 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97926 |
| Spacegroup name | P 64 |
| Unit cell lengths | 95.994, 95.994, 103.120 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.520 - 1.800 |
| R-factor | 0.1583 |
| Rwork | 0.158 |
| R-free | 0.18377 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.273 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.520 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.111 | 0.534 |
| Number of reflections | 49541 | |
| <I/σ(I)> | 31.9 | 5 |
| Completeness [%] | 99.2 | 98.4 |
| Redundancy | 14.5 | 10.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 297 | 0.1M Tris-HCl pH 8.5, 0.2M Na(OAC), 30% PEG 4000, VAPOR DIFFUSION, SITTING DROP, temperature 297K |
