2R89
Crystal structure of the long-chain fatty acid transporter FadL mutant delta N3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X6A |
Synchrotron site | NSLS |
Beamline | X6A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-06-10 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.10 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 92.015, 91.912, 289.190 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 3.400 |
R-factor | 0.252 |
Rwork | 0.252 |
R-free | 0.30200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1t16 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.550 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.810 | 3.520 |
High resolution limit [Å] | 3.400 | 3.400 |
Rmerge | 0.083 | 0.353 |
Number of reflections | 17064 | |
<I/σ(I)> | 8.8 | 3.4 |
Completeness [%] | 99.1 | 98.3 |
Redundancy | 4.3 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 20% PEG 10000, 8% ethylene glycol, 0.1M Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |