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2R7U

Crystal Structure of Rotavirus SA11 VP1/RNA (AAAAGCC) Complex

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 24-ID-C
Synchrotron siteAPS
Beamline24-ID-C
Temperature [K]100
Detector technologyCCD
Collection date2006-02-22
DetectorADSC QUANTUM 315
Wavelength(s)0.97949
Spacegroup nameP 21 21 21
Unit cell lengths76.743, 112.151, 144.307
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution30.000 - 3.100
Rwork0.234
R-free0.28900
Structure solution methodMIRAS
Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwareSHARP
Refinement softwareCNS
Data quality characteristics
 OverallInner shellOuter shell
Low resolution limit [Å]30.00030.0003.110
High resolution limit [Å]3.0006.4403.000
Rmerge0.1050.0290.536
Number of reflections23905
<I/σ(I)>11.9
Completeness [%]92.499.340.9
Redundancy5.15.81.8
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP72851 microliter of VP1 at 10 mg/ml in 25 mM Na-HEPES, pH 7.8, 100 mM NaCl mixed with 2 microliter of crystallization buffer [25 mM Na-MES, pH 6.5, 1.5% (w/v) PEG 3350] and allowing the drop to equilibrate at 12 C by hanging-drop vapor diffusion with a well solution identical in composition to the drop except for the protein. With micro-seeding, thin, plate-like crystals appeared after 1 day and grew to full size in about two weeks., pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 285K
Crystallization Reagents
IDcrystal IDsolution IDreagent nameconcentrationdetails
111Na-HEPES
211NaCl
312Na-MES
412PEG 3350

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