2R7I
Crystal structure of catalytic subunit of protein kinase CK2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 43 |
| Unit cell lengths | 127.517, 127.517, 126.406 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.574 - 3.003 |
| R-factor | 0.2181 |
| Rwork | 0.216 |
| R-free | 0.25710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.934 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.110 |
| High resolution limit [Å] | 2.950 | 3.000 |
| Rmerge | 0.154 | 0.648 |
| Number of reflections | 40213 | |
| <I/σ(I)> | 14.2 | 2.85 |
| Completeness [%] | 99.0 | 100 |
| Redundancy | 7.2 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | Li2SO4, PEG 3350, Bis-Tris buffer, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
