2R78
Crystal structure of a domain of the sensory box sensor histidine kinase/response regulator from Geobacter sulfurreducens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-08-21 |
| Detector | SBC-2 |
| Wavelength(s) | 0.97940 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.248, 62.501, 71.203 |
| Unit cell angles | 90.00, 94.82, 90.00 |
Refinement procedure
| Resolution | 70.890 - 1.600 |
| R-factor | 0.1812 |
| Rwork | 0.179 |
| R-free | 0.22098 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.500 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.890 | 1.642 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.069 | 0.508 |
| Number of reflections | 55938 | |
| <I/σ(I)> | 31.36 | 1.73 |
| Completeness [%] | 99.5 | 95.59 |
| Redundancy | 8.6 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 289 | 0.2M Ammonium acetate, 0.1M Bis-Tris, 25% PEG 3350, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
