2R76
Crystal structure of the rare lipoprotein B (SO_1173) from Shewanella oneidensis, Northeast Structural Genomics Consortium Target SoR91A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4C |
| Synchrotron site | NSLS |
| Beamline | X4C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-08-29 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97893 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 88.091, 88.091, 112.235 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.600 |
| R-factor | 0.247 |
| Rwork | 0.247 |
| R-free | 0.29300 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SnB |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.076 | 0.424 |
| Number of reflections | 25394 | |
| <I/σ(I)> | 40.72 | 5.2 |
| Completeness [%] | 98.6 | 99 |
| Redundancy | 14.6 | 13.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH UNDER OIL | 4.6 | 291 | Protein solution: 10 mM Tris-HCl pH 7.5, 100 mM NaCl, 5 mM DTT. Reservoir solution: 0.1 M Sodium acetate pH 4.6, 15% PEG 20000, MICROBATCH UNDER OIL, temperature 291K |
