2R75
Aquifex aeolicus FtsZ with 8-morpholino-GTP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-20 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.846, 74.177, 44.197 |
| Unit cell angles | 90.00, 95.08, 90.00 |
Refinement procedure
| Resolution | 16.850 - 1.402 |
| R-factor | 0.13936 |
| Rwork | 0.137 |
| R-free | 0.18441 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2r6r |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.840 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.480 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.051 | 0.153 |
| Number of reflections | 51744 | |
| <I/σ(I)> | 13.4 | 4.7 |
| Completeness [%] | 94.1 | 98.1 |
| Redundancy | 2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 292 | 15 g/L FtsZ, 0.1 M Na citrate pH 5.5, 0.1 M NaCl, 0.06 M MgCl2, 25% PEG400, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
