2R73

Crystal Structure of the Possum Milk Whey Lipocalin Trichosurin at pH 8.2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	113
Detector technology	IMAGE PLATE
Collection date	2002-06-03
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5419
Spacegroup name	P 43 21 2
Unit cell lengths	101.156, 101.156, 140.557
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.500
Rwork	0.217
R-free	0.26900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2r74
RMSD bond length	0.006
RMSD bond angle	1.236
Data reduction software	DENZO
Data scaling software	HKL-2000
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.085	0.516
Number of reflections	25922
<I/σ(I)>	33.9	6.4
Completeness [%]	100.0	100
Redundancy	20.24

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.2	291	12% PEG 4000, 200mM LiSO4, 100mM Tris-HCl, pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 291K