2R6X

Structure of a D35N variant PduO-type ATP:co(I)rrinoid adenosyltransferase from Lactobacillus reuteri complexed with ATP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	CCD
Collection date	2007-05-25
Detector	Bruker Platinum 135
Spacegroup name	P 63
Unit cell lengths	64.772, 64.772, 169.438
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.610
R-factor	0.177
Rwork	0.173
R-free	0.24900
RMSD bond length	0.012
RMSD bond angle	1.395
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.660
High resolution limit [Å]	2.610	2.610
Number of reflections	12422
<I/σ(I)>	26.1
Completeness [%]	99.6	99.5
Redundancy	7.5	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6	300	ANOXIC, 16% PEG 8000, 0.1 M MES, 200 mM KCl, 30 ug/mL FMN reductase, 50 mM NADH, 10 mM FMN, 10 mM hydroxycobalamin, 10 mM MgCl2, 10 mM ATP, pH 6.0, vapor diffusion, temperature 300K