2R6S
Crystal structure of Gab protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-07 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.931 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 120.900, 120.900, 137.170 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.360 - 2.100 |
| R-factor | 0.163 |
| Rwork | 0.162 |
| R-free | 0.19900 |
| Starting model (for MR) | 1jr7 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.481 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.400 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.131 | 0.632 |
| Number of reflections | 29931 | |
| <I/σ(I)> | 10.5 | 2.4 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 4.9 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 298 | 1.6M ammonium sulphate, 0.1M bicine, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
