2R6Q
Crystal Structure of BclA-island Construct
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-15 |
| Detector | MarUSA MarMosaic -325 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 67.496, 67.496, 163.933 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 1.430 |
| R-factor | 0.13 |
| Rwork | 0.130 |
| R-free | 0.14700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | BclA(PDB ID: 1WCK) |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.652 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.480 |
| High resolution limit [Å] | 1.430 | 3.080 | 1.430 |
| Rmerge | 0.049 | 0.030 | 0.428 |
| Number of reflections | 41307 | ||
| <I/σ(I)> | 14.3 | ||
| Completeness [%] | 98.9 | 98.8 | 91.6 |
| Redundancy | 19.4 | 19.7 | 10.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | di-Sodium tartrate, vapor diffusion, sitting drop, temperature 295K |
