2R6J
Structure of Eugenol Synthase from Ocimum basilicum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-09-10 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.301, 86.049, 76.440 |
| Unit cell angles | 90.00, 107.71, 90.00 |
Refinement procedure
| Resolution | 33.080 - 1.500 |
| Rwork | 0.205 |
| R-free | 0.22500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qw8 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.310 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 34.120 | 34.120 | 1.580 |
| High resolution limit [Å] | 1.500 | 4.740 | 1.500 |
| Rmerge | 0.104 | 0.069 | 0.398 |
| Total number of observations | 11734 | 9520 | |
| Number of reflections | 87251 | ||
| <I/σ(I)> | 4.5 | 8.2 | 1.5 |
| Completeness [%] | 81.5 | 98 | 34.3 |
| Redundancy | 3.2 | 3.4 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277 | 0.1 M sodium succinate, 21% PEG 3350, 0.3 M KCl, 2 mM dithiothreitol, 5 mM NADP+, pH 5.5, vapor diffusion, hanging drop, temperature 277K |
