2R6H

Crystal structure of the domain comprising the NAD binding and the FAD binding regions of the NADH:ubiquinone oxidoreductase, Na translocating, F subunit from Porphyromonas gingivalis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2006-12-18
Detector	SBC-3
Wavelength(s)	0.97940
Spacegroup name	I 41 2 2
Unit cell lengths	175.681, 175.681, 244.292
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.540 - 2.950
R-factor	0.221
Rwork	0.215
R-free	0.27100
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	0.685
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.540	3.060
High resolution limit [Å]	2.950	2.950
Number of reflections	40721
<I/σ(I)>	5.2	3.7
Completeness [%]	100.0	100
Redundancy	17.5	16.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	289	0.1 M Tris-HCl pH 8.5, 2M Ammonium sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 289K