2R68
Complex Structure of Sucrose Phosphate Synthase (SPS)-S6P of Halothermothrix orenii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-08-06 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 155.399, 48.189, 75.008 |
| Unit cell angles | 90.00, 101.21, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.400 |
| Rwork | 0.209 |
| R-free | 0.26800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2r60 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.297 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.340 |
| High resolution limit [Å] | 2.260 | 4.870 | 2.260 |
| Rmerge | 0.050 | 0.025 | 0.335 |
| Number of reflections | 25868 | ||
| <I/σ(I)> | 15.5 | 2 | |
| Completeness [%] | 99.8 | 99.7 | 98.5 |
| Redundancy | 3.6 | 3.6 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 20% PEG 4000, 0.6M NaCl, 0.1M Na MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
