2R65
Crystal structure of Helicobacter pylori ATP dependent protease, FtsH ADP complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2006-05-17 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.00 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 110.471, 219.485, 146.692 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 3.300 |
R-factor | 0.28972 |
Rwork | 0.288 |
R-free | 0.33058 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ixz |
RMSD bond length | 0.020 |
RMSD bond angle | 1.822 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASES |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.380 |
High resolution limit [Å] | 3.300 | 3.300 |
Rmerge | 0.052 | 0.342 |
Number of reflections | 25338 | |
<I/σ(I)> | 37.9 | 3.2 |
Completeness [%] | 98.4 | 91.9 |
Redundancy | 4.4 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.1M MES-NaOH pH 6.5, 17%(w/v) PEG 8000, 0.2M MgSO4, 0.01M ADP, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |