2R65
Crystal structure of Helicobacter pylori ATP dependent protease, FtsH ADP complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2006-05-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.00 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 110.471, 219.485, 146.692 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.300 |
| R-factor | 0.28972 |
| Rwork | 0.288 |
| R-free | 0.33058 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ixz |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.822 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.380 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.052 | 0.342 |
| Number of reflections | 25338 | |
| <I/σ(I)> | 37.9 | 3.2 |
| Completeness [%] | 98.4 | 91.9 |
| Redundancy | 4.4 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.1M MES-NaOH pH 6.5, 17%(w/v) PEG 8000, 0.2M MgSO4, 0.01M ADP, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
