2R5S
The crystal structure of a domain of protein VP0806 (unknown function) from Vibrio parahaemolyticus RIMD 2210633
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-08-11 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97918, 0.97938 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 53.001, 116.476, 62.224 |
| Unit cell angles | 90.00, 88.75, 90.00 |
Refinement procedure
| Resolution | 29.120 - 2.140 |
| R-factor | 0.22651 |
| Rwork | 0.223 |
| R-free | 0.27922 |
| Structure solution method | MAD |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.073 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.120 | 2.200 |
| High resolution limit [Å] | 2.140 | 2.140 |
| Rmerge | 0.059 | 0.378 |
| Number of reflections | 19762 | |
| <I/σ(I)> | 28.23 | 2.47 |
| Completeness [%] | 95.5 | 77.3 |
| Redundancy | 3.4 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 289 | 20% PEG 8000, 0.1M MES, 0.2M Ca(OAc)2, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
