2R5O
Crystal structure of the C-terminal domain of wzt
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-10 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 106.081, 106.081, 70.351 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.300 |
| R-factor | 0.167 |
| Rwork | 0.166 |
| R-free | 0.18200 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.456 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE (2.10) |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.350 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Number of reflections | 98154 | |
| <I/σ(I)> | 16.5 | |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 6.9 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 24% PEG 5000 MME, 6% (v/v) glycerol, 0.4 M NaCl, 0.16 M (NH4)2SO4, 0.08 M Na Acetate, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
