2R5M
Crystal Structure of the two MBT repeats from Sex-Comb on Midleg (SCM) in complex with peptide R-(me)K-S
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.934 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 82.710, 82.710, 76.230 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.650 |
| R-factor | 0.17295 |
| Rwork | 0.169 |
| R-free | 0.24467 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.303 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.550 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.101 | 0.368 |
| Number of reflections | 9984 | |
| <I/σ(I)> | 4.1 | 1.4 |
| Completeness [%] | 97.9 | 99 |
| Redundancy | 13.3 | 13.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.3 | 293 | 15% (w/v) PEG3350, 100 mM calcium acetate and 3% ethanol, pH 7.3, VAPOR DIFFUSION, temperature 293K |
