2R5G
Structure of human CLIC2, crystal form B
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2007-04-10 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 35.973, 66.940, 44.071 |
Unit cell angles | 90.00, 99.86, 90.00 |
Refinement procedure
Resolution | 20.650 - 1.860 |
R-factor | 0.18617 |
Rwork | 0.184 |
R-free | 0.22764 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1k0m |
RMSD bond length | 0.007 |
RMSD bond angle | 1.065 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.700 | 1.950 |
High resolution limit [Å] | 1.860 | 1.860 |
Rmerge | 0.047 | 0.315 |
Number of reflections | 17172 | |
<I/σ(I)> | 24.2 | 2.4 |
Completeness [%] | 97.7 | 84.3 |
Redundancy | 6 | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 30%-32% PEG 400, 100mM Tris-HCl, pH7.5, 5mM GSH, VAPOR DIFFUSION, HANGING DROP, temperature 293K |