2R5G
Structure of human CLIC2, crystal form B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-04-10 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.973, 66.940, 44.071 |
| Unit cell angles | 90.00, 99.86, 90.00 |
Refinement procedure
| Resolution | 20.650 - 1.860 |
| R-factor | 0.18617 |
| Rwork | 0.184 |
| R-free | 0.22764 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1k0m |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.065 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.700 | 1.950 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Rmerge | 0.047 | 0.315 |
| Number of reflections | 17172 | |
| <I/σ(I)> | 24.2 | 2.4 |
| Completeness [%] | 97.7 | 84.3 |
| Redundancy | 6 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 30%-32% PEG 400, 100mM Tris-HCl, pH7.5, 5mM GSH, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
